Selective translation initiation on bicistronic simian virus 40 late mRNA
نویسندگان
چکیده
منابع مشابه
Stimulation of simian virus 40 late gene expression by simian virus 40 tumor antigen.
The early simian virus 40 (SV40) gene product, large tumor (T) antigen, is responsible for the initiation of viral DNA replication and the autoregulation of early gene expression through direct protein-DNA interactions. We investigated the role of T antigen in late viral gene expression, independent of its function in amplifying templates through DNA replication. SV40 DNA was transfected into B...
متن کاملSequences locating the 5' ends of the major simian virus 40 late mRNA forms.
The 5' sequences of late mRNA specified by several constructed or naturally occurring deletion or duplication mutants of simian virus 40 were examined. The mutants included viruses with various small deletions centered about 25 nucleotides upstream from the major transcription initiation site, as well as viruses containing tandem duplications of a sequence of 50 nucleotides or less embedding th...
متن کاملSpecific initiation site for simian virus 40 deoxyribonucleic acid replication.
Replicating simian virus 40 (SV40) deoxyribonucleic acid (DNA) molecules have been isolated under conditions in which the newly synthesized DNA is uniformly labeled with (3)H-thymidine. These newly synthesized strands are released from the replicative intermediate molecules by alkaline treatment, and it has been possible to isolate single-stranded SV40 DNA which varies in size from 157,000 dalt...
متن کاملTranslation of equine infectious anemia virus bicistronic tat-rev mRNA requires leaky ribosome scanning of the tat CTG initiation codon.
We have examined the translational regulation of the equine infectious anemia virus (EIAV) bicistronic tat-rev mRNA. Site-directed mutagenesis of the tat leader region followed by expression of the tat-rev cDNA both in vitro and in transiently transfected cells established that tat translation is initiated exclusively at a CTG codon. Increasing the efficiency of tat translation by altering the ...
متن کاملProteins in intracellular simian virus 40 nucleoportein complexes: comparison with simian virus 40 core proteins.
Intracellular nucleoprotein complexes containing SV40 supercoiled DNA were purified from cell lysates by chromatography on hydroxyapatite columns followed by velocity sedimentation through sucrose gradients. The major protein components from purified complexes were identified as histone-like proteins. When analyzed by electrophoresis in sodium dodecyl sulfate-polyacrylamide gels, complex protei...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1987
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.61.7.2331-2335.1987